LABORATORY

 

Live Foods:

In most aquaculture operations which involve larval culture it is essential that the laboratory associated with the facility have a good Live Foods room(s).  Live Foods includes unicellular microalgae (many species available of non-motile, diatom, flagellates), rotifers (Branchionus spp) and brine shrimp (Artemia salina)

Microalgae:

All commercial bivalve molluscs require microalgae which can be maintained and upscaled from an Algal Room.  Throughout the larval life cycle there are changes in microalgae diets for many bivalve molluscs and the hatchery manager must be aware of the needs and requirements of each species of bivalve mollusc being cultured [e.g. scallops, pearl oysters, rock oysters, mussels, clams].  Thus far, microencapsulated diets or frozen microalgae do not satisfy the dietary requirements of most bivalve molluscs, so live microalgae must be included as a part of the diet.  Aquasearch has feeding schedules for the days of the larval life of blacklip pearl oysters, giant clams, and scallops.   These are provided as part of a consultancy to setup a hatchery and in training hatchery technicians.  

Aquasearch has its own cheap algal culture media.  This has been tested for several years and serves as an example for setting up a good quality but economically affordable Algal Culture Room in third world countries.  One medium is used for the stock microalgae cultures in the Algal area using reduced natural light and another is used for the mass microalgae cultures which can be grown outdoors using natural sunlight.   However, when consulting to set up new hatcheries the standard f2 medium and durable culture vessels are used if the client has the appropriate budget. 

The following is a diagram showing a General Protocol for Culturing Microalgae:

Rotifers:

The marine rotifer Branchionus plicatilis is an important live food, as it is the first food which can be eaten by many marine fish, and some crustaceans such as the mudcrab, Scylla serrata.    They generally reproduce asexually (parthenogenesis) but when environmental stress appears they can switch to a sexual mode of reproduction.  A general morphology of a branchionid rotifer is shown below:

lorica,corona (trochal disc),eyespot,ovary,mastax,stomach,gastric gland,flame bulb,bladder,intestine,foot,toe,vitellarium

 

 

 

 

 

 

 

 

 

 

The body size of the seawater variety is about 120 microns diameter by 250 microns long.  There are two strains that are most used in aquaculture; the large or L strain (0.33 micrograms / rotifer) and the small or S strain (0.22 micrograms / rotifer).  They grow best at salinities of 10-20 ppt and the light level should be 2000-5000 lux at a pH of 7.5-8.5.   Rotifers can be cultured in continuous, semi-continuous, and batch cultures. Yields can be over 500 rotifers/ml. 

The food most often used for the rotifers is Nannochloropsis oculata, but it has been found that higher yields are obtained by using Tetraselmis spp. or combinations of these with diatoms.  Yeast can be fed as a supplemental food but it often has to be enhanced with nutrients and vitamins and it can rapidly pollute the culture. 

 

Brine Shrimp:

Artemia is a crustacean which can be hatched at will from stored dry cysts.  The nauplii larvae which are newly hatched have the highest nutritional value for feeding to the target aquacultured larvae.  It has been estimated that approximately 85% of cultured marine species will feed on Artemia.  

Since commercial dry cysts are relatively expensive, hatcheries on small budgets may consider maintaining breeding adults which happily produce eggs that hatch directly to nauplii rather than producing cysts.  Artemia feed on microalgae much as rotifers do, so it is possible to maintain broodstock and routinely collect nauplii larvae for feeding to target aquaculture larvae. 

 

 

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Updated 1 January 2015; Copyright Aquasearch